Generate a synthetic cr_experiment

Creates a realistic synthetic cell-culture microscopy data set for demonstration and testing. The simulated experiment has six treatment groups, four replicates per group and 96 wells in total. Four fluorescence channels are generated: a nuclear stain (DAPI), a damage marker (marker_1), a viability marker (marker_2) and a secondary readout (marker_3).

Usage

cr_example_experiment(
  seed = 42,
  n_cells_per_well = 150,
  n_wells_per_replicate = 4
)

Arguments

seed

Random seed.

n_cells_per_well

Mean number of cells per well (Poisson).

n_wells_per_replicate

Number of wells per replicate.

Value

A cr_experiment.

Examples

exp <- cr_example_experiment(seed = 1, n_cells_per_well = 20)
print(exp)
#> ── cr_experiment ───────────────────────────────────────────────────────────────
#> • Cells: 1961 across 96 wells
#> • Channels: "DAPI", "marker_1", "marker_2", and "marker_3"
#> • Design: 6 treatment groups
#> • QC steps applied: 0
#> ℹ Metadata fields: project and sop