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Annotations are only useful if they can leave the tool that made them. hyperannotatR stores every region of interest as a validated simple-feature geometry in image pixel coordinates, with an explicit pyramid level attached, which makes it straightforward to hand work off to a viewer such as QuPath, to a GIS-flavoured GeoJSON consumer, or to a Python segmentation pipeline, and to take the results back again. Because the geometry model is the same one used internally, an export followed by an import is a genuine round-trip rather than a lossy approximation, and this vignette shows a live one for each format so you can see that labels, identities, and coordinates survive intact.

A guiding principle runs through all four formats below: the coordinate convention never changes silently. Coordinates are written in image pixels unless you deliberately ask otherwise, so a polygon you exported lands back on exactly the same pixels when you read it in.

We use the bundled example project throughout: three labelled rectangles on a small tissue raster.

proj <- at_example_project()
at_rois(proj)[, c("roi_id", "layer", "label", "geom_type", "area_px")]
#> # A tibble: 3 × 5
#>   roi_id        layer   label    geom_type area_px
#>   <chr>         <chr>   <chr>    <chr>       <dbl>
#> 1 roi_000000001 regions tumour   POLYGON     19600
#> 2 roi_000000002 regions necrosis POLYGON     72704
#> 3 roi_000000003 regions stroma   POLYGON     34200

GeoJSON

at_write_geojson() serialises a project to a standard FeatureCollection. Each ROI becomes one Feature whose geometry is the pixel-coordinate polygon and whose properties carry the full hyperannotatR schema: roi_id, layer, label, level (the level of the written coordinates), source_level, coordinate_schema, author, created, modified, source, the writing annotatR_version, and any free-form attributes. Keeping the schema explicit is what lets a file survive a return trip.

gj <- tempfile(fileext = ".geojson")
at_write_geojson(proj, gj)
cat(readLines(gj)[1:22], sep = "\n")
#> {
#>   "type": "FeatureCollection",
#>   "features": [
#>     {
#>       "type": "Feature",
#>       "id": "roi_000000001",
#>       "geometry": {
#>         "type": "Polygon",
#>         "coordinates": [
#>           [
#>             [120, 150],
#>             [260, 150],
#>             [260, 290],
#>             [120, 290],
#>             [120, 150]
#>           ]
#>         ]
#>       },
#>       "properties": {
#>         "roi_id": "roi_000000001",
#>         "layer": "regions",
#>         "label": "tumour",

The roi_id is preserved verbatim, so an ROI keeps its identity across the boundary and you can join exported measurements back to the original annotation. Reading the file back with at_read_geojson() reconstructs one annot_layer per distinct layer property. We rebuild a project on the original image and check that the labels came through intact.

back <- at_read_geojson(gj)
imported <- at_project(proj$image, back[["regions"]])
identical(sort(at_rois(proj)$label), sort(at_rois(imported)$label))
#> [1] TRUE

Coordinate levels and legacy imports

An ROI can be stored at any level of a pyramidal image, and the writers’ level argument transforms the coordinates to another level on the way out. GeoJSON and QuPath exports write level matching their coordinates, with the stored ROI level in source_level as provenance. The coordinate_schema = "annotatR-pixel-level-v1" marker identifies this contract. CSV exports always use level-0 coordinates and level = 0. Image dimensions determine separate x and y scale factors; no power-of-two pyramid is inferred. Vector geometry round trips use floating-point precision (relative tolerance 1e-12), so a region drawn on an overview level comes back with the same level-0 area whichever format carried it:

mx <- at_example_image("multiplex")
lp <- at_project(mx, at_layer_add(at_layer("regions"),
                                  at_roi_rect(10, 10, 40, 40, label = "a", level = 1L)))
gj1 <- tempfile(fileext = ".geojson")
at_write_geojson(lp, gj1)
csv1 <- tempfile(fileext = ".csv")
at_write_rois_csv(lp, csv1)
c(stored  = at_rois(lp)$area_px,
  geojson = at_rois(at_project(mx, at_read_geojson(gj1)[["regions"]]))$area_px,
  csv     = at_rois(at_project(mx, at_read_rois_csv(csv1, layer_name = "regions")))$area_px)
#>  stored geojson     csv 
#>    3600    3600    3600

Some older exports, including GeoJSON, CSV and QuPath files written by hyperannotatR 0.2.0 and earlier, paired level-0 geometry with a nonzero stored ROI level. Those files cannot be distinguished reliably from valid nonzero-coordinate files, so readers reject an unmarked nonzero recorded level by default with an AMBIGUOUS_LEVEL error. When the actual coordinate level is known, pass it as coordinate_level (for example 0L for such hyperannotatR exports) to at_read_geojson(), at_read_rois_csv() or at_read_qupath(); this overrides the level declaration without scaling coordinates. To trust the recorded levels of legitimate foreign or legacy files, pass legacy_levels = "recorded". Use either only when the coordinate convention is known; never guess a repair. Files without level metadata default to level 0, or to the reader’s fallback level.

Mask imports, including NPY, recover the level from the RDS object or the legend sidecar by default; an explicit level overrides it. Keep the sidecar next to the raster, and for external masks without metadata supply the level when it is not 0.

The flip_y option

hyperannotatR’s convention is image orientation: the origin is top-left and y increases downward. Many GIS tools instead expect y to increase upward. Set flip_y = TRUE to write coordinates in that upward convention; the y-axis is mirrored about the image height. If you write flipped, you must read flipped, supplying the same height so the inversion is undone:

h <- at_dims(proj$image)[2]
gj_flip <- tempfile(fileext = ".geojson")
at_write_geojson(proj, gj_flip, flip_y = TRUE)
regained <- at_read_geojson(gj_flip, flip_y = TRUE, height = h)
identical(sort(at_rois(at_project(proj$image, regained[["regions"]]))$centroid_y),
          sort(at_rois(proj)$centroid_y))
#> [1] TRUE

Leave flip_y at its default FALSE when exchanging with other image-coordinate tools (including QuPath and hyperannotatR itself); reach for it only when a downstream consumer explicitly wants a cartesian, bottom-left origin.

QuPath

QuPath reads and writes its own GeoJSON dialect. By default at_write_qupath() writes the form QuPath 0.4 and later read natively, checked against a real QuPath 0.7.0 installation: objectType (annotation, or detection for mask-derived ROIs), a classification with its name (or the names of a derived class such as "Tumor: Positive") and an [r, g, b] color, and isLocked for locked ROIs. QuPath silently replaces feature ids that are not UUIDs, so each ROI gets a deterministic UUID (existing UUIDs are kept). The feature properties carry level, source_level and coordinate_schema, but QuPath keeps only properties.metadata through its own import and export, so hyperannotatR repeats what it needs there as strings: annotatr_roi_id, annotatr_layer, annotatr_source, annotatr_level (the stored ROI level, as in hyperannotatR 0.2.0), annotatr_coordinate_schema and annotatr_coordinate_level. QuPath 0.7.0 keeps these entries, so the original id and the coordinate level come back; if another tool drops the metadata, a non-UUID id cannot be recovered from the UUID.

at_read_qupath() accepts a native feature array (QuPath’s default export), a FeatureCollection or a single Feature, in the current dialect or the legacy object_type/colorRGB form. It restores labels, colours and ids, and keeps everything else QuPath carries under attributes$qupath: object type, measurements with explicit NaN/infinity states, image planes, cell nuclei, and a flag on ellipses that QuPath exported as polygons. Features without a classification are imported as "unclassified" rather than dropped. Input is validated strictly: malformed structure, contradictory repeated fields (identical repeats, which QuPath 0.7.0 emits, are accepted), conflicting colours or object types, and repeated ROI ids are refused with an INVALID_GEOJSON error.

qp <- tempfile(fileext = ".geojson")
at_write_qupath(proj, qp)
feature <- jsonlite::read_json(qp)$features[[1]]
str(feature$properties[c("objectType", "classification", "metadata")], max.level = 2)
#> List of 3
#>  $ objectType    : chr "annotation"
#>  $ classification:List of 2
#>   ..$ name : chr "tumour"
#>   ..$ color:List of 3
#>  $ metadata      :List of 6
#>   ..$ annotatr_roi_id           : chr "roi_000000001"
#>   ..$ annotatr_layer            : chr "regions"
#>   ..$ annotatr_source           : chr "manual"
#>   ..$ annotatr_level            : chr "0"
#>   ..$ annotatr_coordinate_schema: chr "annotatR-pixel-level-v1"
#>   ..$ annotatr_coordinate_level : chr "0"

The colour gotcha

Older QuPath versions, and hyperannotatR 0.1, stored a classification colour as a signed 32-bit colorRGB integer packed as 0xFFRRGGBB. The 0xFF alpha byte pushes most colours past 2^31, so they wrap around to negative values: the grey #999999 serialises as -6710887. hyperannotatR still reads that form and writes it with dialect = "legacy"; the modular arithmetic is handled in both directions. QuPath keeps a single colour per classification name, so identical labels with different layer colours collapse to the first colour it sees. Reading the file back recovers the hex colours onto the layer style:

lyr <- at_read_qupath(qp)
lyr$style$colour
#>    tumour  necrosis    stroma 
#> "#999999" "#E69F00" "#56B4E9"

Masks for Python

For pixel-level pipelines the exchange currency is a raster mask, not a polygon. at_write_mask() writes an integer TIFF alongside a self-describing <path>.legend.json sidecar that records the mask type, level, dimensions, and one entry per class (value, label, layer, roi_id, n_px, colour). The sidecar is what turns an anonymous grid of integers into something a downstream model can interpret without guessing which value meant which class, and it travels with the raster so the mapping is never lost.

m <- at_mask(proj, type = "labelled")
mp <- tempfile(fileext = ".tiff")
at_write_mask(m, mp)
cat(readLines(paste0(mp, ".legend.json"))[1:14], sep = "\n")
#> {
#>   "mask_metadata": {
#>     "schema_version": 1,
#>     "status": "declared",
#>     "encoding": "instance",
#>     "background": 0,
#>     "overlap": "last",
#>     "grid": {
#>       "dims": [512, 512],
#>       "level": 0,
#>       "origin": [0, 0],
#>       "stride": [1, 1]
#>     },
#>     "source": {

Here we used a "labelled" mask, where each ROI gets its own integer; a "binary" mask collapses everything to foreground and background, and a "multiclass" mask shares one integer per label. Whichever you choose, the legend describes exactly what the pixels mean. On the Python side, read the TIFF as an integer array and load the legend to map values back to labels. This chunk is illustrative and is not run when the vignette is built.

import json
import numpy as np
import tifffile

mask = tifffile.imread("mask.tiff")
with open("mask.tiff.legend.json") as f:
    legend = json.load(f)

labels = {e["value"]: e["label"] for e in legend["legend"]}
for value, label in labels.items():
    print(label, int(np.sum(mask == value)), "px")

CSV and WKT

For spreadsheet users and pipelines that would rather not parse JSON, at_write_rois_csv() produces one row per ROI with the geometry in a Well-Known Text geometry column, plus the metadata fields. at_read_rois_csv() parses any CSV with at least label and geometry columns back into a layer.

csv <- tempfile(fileext = ".csv")
at_write_rois_csv(proj, csv)
lyr_csv <- at_read_rois_csv(csv, layer_name = "regions")
at_rois(at_project(proj$image, lyr_csv))[, c("label", "geom_type", "area_px")]
#> # A tibble: 3 × 3
#>   label    geom_type area_px
#>   <chr>    <chr>       <dbl>
#> 1 tumour   POLYGON     19600
#> 2 necrosis POLYGON     72704
#> 3 stroma   POLYGON     34200

Because WKT is understood by PostGIS, sf, Shapely, and most spatial databases, the CSV is often the shortest path into a wider geospatial stack.

Export destinations and receipts

Batch and split-mask exports (at_export_all(), at_write_masks() and the app’s export) turn project, layer, ROI and class names into safe, portable filename components and append deterministic suffixes to collisions, including names that differ only in case. Display labels stay in receipts and in the annotation and legend metadata. Exports preflight the complete bundle, including sidecars and receipt files, and reject symlinked paths inside the destination before writing. An explicitly chosen single-file output path, as in at_write_geojson(), can point to any directory.

A mask and its legend are staged before either is published. Existing bundles are skipped unless replacement was requested, and a failed item does not stop later items. The receipt has a row for every requested image and format, with status ok, error or skipped, a message, the paths, and byte counts for the output and its sidecar; ok means the whole bundle was published. A failed publication restores the files it replaced. Session exports rewrite _export_manifest.csv and _annotation_summary.csv on every run; a failure to write them warns and is kept in the receipt’s attributes without discarding the item results.

Serialise exports and keep the destination stable while they run: preflight and several renames are not a race-free transaction against concurrent writers, crashes between renames, or symlinks changed during the run.

For the complete list of import and export helpers, see the reference index on the package website.