Generate an example experimental design

Builds the unit-level design table used by cr_example_experiment(). Six treatment levels are crossed with two plates and two pre-treatment intervals, so that a batch is the combination of plate and interval rather than a single column and every batch still contains untreated units to standardise against.

Usage

cr_example_design(plate_format = 96, n_wells_per_replicate = 4)

Arguments

plate_format

Either 96 or 384.

n_wells_per_replicate

Number of units per replicate per treatment level.

Value

A tibble with one row per unit and the columns well, treatment, dose, dose_unit, group, replicate, plate, interval and timepoint.

Details

Treatment names are deliberately abstract. Untreated is the vehicle level, PosControl a positive control, and CompoundA appears at a low and a high exposure level so that a dose axis exists; CompoundB and CompoundC are further compounds at the low exposure level. The four distinct values of dose are the four exposure levels of the demonstration assay.

See also

cr_example_experiment(), cr_example_screen().

Other example data: cr_example_experiment(), cr_example_exports(), cr_example_files(), cr_example_path(), cr_example_screen()

Examples

design <- cr_example_design(96)
table(design$treatment, design$interval)
#>                 
#>                  15min 60min
#>   CompoundA_high     8     8
#>   CompoundA_low      8     8
#>   CompoundB          8     8
#>   CompoundC          8     8
#>   PosControl         8     8
#>   Untreated          8     8